The level opens with many Pikmin are seen pecking at something, which a change in camera angle reveals to be a giant R.O.B. in a dormant state. Further changes in view reveal hundreds of Pikmin attacking it. The R.O.B. activates and spins its torso, sending all but a few Pikmin to their deaths upon contact with the ground. The camera cuts to show a terrified-looking Olimar on the ground nearby, surrounded by about a dozen Pikmin. One of them, a red one, taps Olimar on the leg to get his attention and points behind him.
Olimar turns to see the Blue Falcon racing down between ruined columns. Suddenly, the vehicle stops and its hatch pops open, sending its occupant flying forward. With a shout of "FALCON PUNCH!!", the camera shows Captain Falcon performing the signature move of the same name. The punch lands between the giant R.O.B.'s eyes, knocking it over and defeating it. Captain Falcon's momentum sends him sliding along the ground, accidentally killing all of Olimar's Pikmin except the single red one at his side. The Captains team up for the level at this point.
After a section of the stage, another cutscene shows the new duo arriving at a cliff overlooking the great ocean of the World of Trophies. They see a flying platform, guarded by Primid, that also holds the trophy of Donkey Kong in chains. The camera cuts to show an Arwing flying after the platform, flown by Falco but also containing a very cramped Diddy Kong. Falco loops and opens the cockpit, allowing Diddy to drop out of the Arwing. To keep himself aloft in the air, he fires up his Rocketbarrel Pack. He then fires his Peanut Popguns at the Primid on the platform, sending them flying. Seeing this action, Captain Falcon smiles, grabs the once again terrified Olimar, and jumps off the cliff towards the platform.
Diddy Kong then lands on the platform, and immediately touches the base of Donkey Kong's trophy. The revived DK breaks out of his chains. The Captains land on the platform next to the ape buddies. More Primid are seen encroaching on the platform, at which point the four team up to drive them off.
After defeating the Subspace Army enemies, the Kongs celebrate their reunion. Falco flies off, giving a thumbs-up, to which Diddy waves his thanks. The platform flies into a hangar on the Isle of the Ancients to close the level, and Donkey Kong, Olimar and Captain Falcon join the player's team.
- R.O.B. Sentry
- R.O.B. Blaster
- R.O.B. Launcher
- Scope Primid
- Sword Primid
- Metal Primid
- Towtow (Great Maze Only)
- Ticken (Great Maze Only)
- Quark Mines (Great Maze Only)
- While the Pikmin are attacking the giant R.O.B., during Olimar's introduction and the first half of the level, Main Theme (Pikmin) plays.
- During Captain Falcon's entrance, Mute City plays.
- During the second half of the level, Fire Field plays.