User blog comment:Wachowman/Who Will Survive SSB5?/@comment-24529455-20141018233754/@comment-32390199-20141021085842

However aside from the palette swaps you can easily see it is a female duo to the color of its eyes. Female Jigglypuffs have the same color eyes as SSB Jigglypuff whereas a male Jigglypuff has a different color. Just like how a female Pikachu has heart-shaped tail and male Pikachu not.